Technical classes

The classes are intended for investigators that are already familiar and feel comfortable with culturing mammalian cells in tissue culture. Classes last for two consecutive half-days and cover all standard culturing techniques including passaging, freezing, thawing and maintaining human ES and iPSC lines, both on the feeders and feeder-free systems. The price is $225 per day ($112 for NINDS funded NU researchers)

We also offer private trouble-shooting sessions for those who are already working with the human stem cells upon request.

Please contact Ljuba Lyass to sign up.


“Ljuba is a wonderful administrator who provides stem cell training. Training side-by-side with her is the best way to develop relevant skills.”

 – Miho Tanaka-Matakatsu,PhD, Dr. Matsuoka lab, Dept. of Otholaringology

During the training I learned the basic protocol, medium formulations, passaging the cells, freezing the cells, when to feed or change the feeder layer, and cleaning the cultures of differentiated cells. The cleaning of the cultures, in my opinion is one the harder techniques to master. With Ljuba’s assistance I feel comfortable in this technique. Ljuba showed me the technique and then allowed me to perform the same procedure. This was performed throughout the entire process. On the final day I went through the entire procedure by myself with her supervision

– Paul Hoover, Skin Disease Center

“The training session teaches basic techniques on culturing hES and hiPS cells. Ljuba is extremely knowledgeable and helpful. She took the time to start with basic principles and techniques. She provided plenty of hands-on practice opportunities, which I found very beneficial for me to gain a deeper and lasting understanding. It was also a very interactive training experience. This is a great class for getting a solid foundation on the basics”

– Zhong Xie, PhD, Research Assistant Professor, Physiology

“The stem cell class is a fantastic resource for learning to work with stem cells. In just a few sessions, Ljuba covered all the major components of stem cell culture and manipulation in a friendly and educational environment. The hands-on practice was especially useful, and the small class size allowed for a lot of personal attention!”

– Nicholas Stephanopoulos, PhD, Simpson Querrey Inst. In BioNanotechnology

“The ESC/iPSC core training session gave me hands on experience with the techniques I needed to jumpstart my thesis work. The expert supervision and technical resources of the facility were invaluable while initiating my investigation and continue to be of great assistance”

– Anil Wadhwani, MSTP student, Neurology, Dr. Kessler lab